Simple Staining for Heat-Fixed Brain Sections after Topographical pH Analysis or Autoradiographic Studies | Zendy

N. A. Parkinson | Zendy; Georgia Christofi | Zendy; Tihomir P. Obrenovitch | Zendy; L. Symon | Zendy

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Simple Staining for Heat-Fixed Brain Sections after Topographical pH Analysis or Autoradiographic Studies

Author(s) -

N. A. Parkinson,

Georgia Christofi,

Tihomir P. Obrenovitch,

L. Symon

Publication year - 1988

Publication title -

journal of cerebral blood flow and metabolism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.167

H-Index - 193

eISSN - 1559-7016

pISSN - 0271-678X

DOI - 10.1038/jcbfm.1988.147

Subject(s) - staining , brain tissue , fluorescence , simple (philosophy) , pathology , chemistry , biology , neuroscience , medicine , optics , physics , philosophy , epistemology

Powerful topographic techniques are now available, among which autoradiographic and fluorescent mapping are the most prevalent. These techniques produce images that usually do not correlate with brain anatomy; subsequent staining is required to allow a precise association between the parameter(s) investigated and brain structures. A simple staining procedure is described, compatible with heat-fixed brain sections, and that was found particularly valuable in association with topographical tissue pH studies using the fluorescent pH indicator umbelliferone.

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