Simultaneous Determination of Local Cerebral Glucose Utilization and Blood Flow by Carbon-14 Double-Label Autoradiography: Method of Procedure and Validation Studies in the Rat

Validation studies were undertaken to establish a computer-assisted double-label autoradiographic strategy employing [ 14 C]2-deoxyglucose ([ 14 C]2DG) and [ 14 C]iodoantipyrine ([ 14 C]IAP) to measure local CMR glu (LCMR glu ) and CBF (LCBF). An organic solvent was used to extract the majority of IAP between first and second film exposures. In contrast to previously published data, all solvents tested produced partial losses of 2DG from tissue, and all allowed 2–6% of IAP to persist even after 5-day washes. Technical-grade chloroform permitted equal retention of unmetabolized and metabolized 2DG. A linear model was established, which was insensitive to the changes in tissue self-absorption that were shown to occur with chloroform extraction. Propagated error in computing tissue [ 14 C]2DG and [ 14 C]IAP was reduced by maximizing IAP extraction (by longer solvent wash times) and by administering 2.5 times as much IAP as 2DG. Fractional 2DG retention was measured in single-label 2DG sections placed on the films, and fractional IAP retention was evaluated by an optimization procedure. With this strategy, double-label values for LCMR glu and LCBF in anesthetized rats agreed with values obtained in matched single-label series to within 5%. The coefficients of variation for the double- and single-label LCMR glu data were virtually identical, whereas the coefficient of variation for double-label LCBF was 1.8 times that of single-label LCBF. The double-label strategy permitted pixel-by-pixel measurement and video display of the LCMR glu /LCBF ratio; the mean value among structures was 0.472 μmol/ml. With proper attention to methodological detail, this double-label strategy shows great promise for routine laboratory application.