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A solvent washout technique is evaluated that could be used to remove one  14 C tracer after a combined autoradiographic CMRglu and CBF study using [ 14 C]2-deoxyglucose (2DG) and [ 14 C]iodoantipyrine (IAP). The technique permits the simultaneous measurement of local CBF and local CMRglu in the same animal without the use of the short-lived tracers of iodine,  123 I and  131 I, for iodoantipyrine labeling. This report shows that brain tissue sections did not retain  14 C from [ 14 C]2DG when chloroform was used as the washout solvent. Chloroform washing removes nearly all the  14 C from [ 14 C]IAP. For this study, careful attention was given to the control, pre-wash measurement of  14 C concentration.

Evaluation of a Double-Tracer Autoradiographic Technique for the Measurement of Both Local Cerebral Glucose Metabolism and Local Cerebral Blood Flow