Open AccessExpression of the multidrug resistance-associated protein (MRP) gene in non-small-cell lung cancer
Author(s) -
Eiichiro Ota,
Yoshiyuki Abe,
Y Oshika,
Yuichi Ozeki,
Masayuki Iwasaki,
Hiroshi Inoué,
Hitoshi Yamazaki,
Yoshito Ueyama,
Koichi Takagi,
Tetsuya Ogata
Publication year - 1995
Publication title -
british journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1995.372
Subject(s) - adenocarcinoma , lung cancer , cancer research , multiple drug resistance , biology , gene expression , carcinoma , etoposide , epidermoid carcinoma , vindesine , reverse transcription polymerase chain reaction , cancer , pathology , chemotherapy , medicine , gene , drug resistance , biochemistry , vincristine , microbiology and biotechnology , cyclophosphamide
We examined the levels of expression of the multidrug resistance-associated protein (MRP) gene quantified by Northern blot analysis in comparison with those of the MDR1 gene determined by reverse transcription-polymerase chain reaction (RT-PCR) in 104 non-small-cell lung cancer (NSCLC) specimens [59 adenocarcinoma (Ad), 40 squamous cell carcinoma (Sq), four large cell carcinoma (La) and one adeno-squamous carcinoma (AdSq)]. Thirty-three (31.7%) of the 104 NSCLC expressed the MRP gene at various levels. The NSCLC showing high (++) levels of MRP gene expression (19 out of 33, 57.6%) were predominantly squamous cell carcinomas (Ad, 5; Sq, 13; La, 1) (P < 0.05). Six of the eight NSCLCs expressing high levels of MRP mRNA and no MDR1 (MRP ++, MDR1-) were squamous cell carcinomas. Sixty-one of the 104 NSCLC patients received chemotherapy with MRP-related anti-cancer drugs [vindesine (VDS) and etoposide (VP-16)]. Twenty-three patients (37.7%) with tumour expressing high or moderate levels of MRP showed significantly worse prognoses than those with non- or low-MRP-expressing tumours (P < 0.05). These results suggest that the level of MRP gene expression is related to the histopathology and prognosis of NSCLC.