Open AccessHigh-affinity binding sites for gastrin-releasing peptide on human colorectal cancer tissue but not uninvolved mucosa
Author(s) -
Shaun R. Preston,
Woodhouse Lf,
S Jones-Blackett,
GV Miller,
JN Primrose
Publication year - 1995
Publication title -
british journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1995.210
Subject(s) - bombesin , gastrin releasing peptide , colorectal cancer , radioligand , receptor , peptide , gastrin , cancer , medicine , endocrinology , cancer research , chemistry , biology , neuropeptide , biochemistry , secretion
Human colorectal cancer tissue and matched uninvolved mucosa from 21 patients were examined by radioligand displacement for the presence of binding sites for bombesin-like peptides. Five cancers, but no uninvolved mucosa, expressed high-affinity, low-capacity bombesin binding sites (Kd = 6.53 nM, Bmax = 58.6 fmol mg-1 protein) of the gastrin-releasing peptide (GRP)-preferring subtype (IC50 4.8 nM). Bombesin-like peptides may have a role in the pathogenesis of colorectal cancer, and bombesin receptor antagonists may be of value in the treatment of receptor-positive tumours.