Open AccessCharacterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection
Author(s) -
Lemoine Nr,
Mayall Es,
Travis H. Jones,
Denise Sheer,
S McDermid,
P. KendallTaylor,
David WynfordThomas
Publication year - 1989
Publication title -
british journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1989.387
Subject(s) - transfection , thyroglobulin , cell culture , biology , thyroid , in vitro , microbiology and biotechnology , virus , epithelium , cancer research , virology , endocrinology , genetics
Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.