Open AccessThyroid epithelial cell transformation by a retroviral vector expressing SV40 large T
Author(s) -
Jorge S. Burns,
Louise Lemoine,
Lemoine Nr,
Williams Ed,
David WynfordThomas
Publication year - 1989
Publication title -
british journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1989.158
Subject(s) - viral vector , transfection , biology , recombinant dna , cell culture , virology , thyroid , virus , vector (molecular biology) , phenotype , antigen , cancer research , microbiology and biotechnology , expression vector , transformation (genetics) , gene , immunology , genetics
A recombinant murine retroviral vector encoding the SV40 virus large T antigen was used to infect stably an immortal line of differentiated rat thyroid epithelial cells, FRTL-5. Expression of SV40 T transformed these cells to anchorage independence and tumorigenicity but did not alter morphology or abolish tissue-specific functions and growth factor requirements. The resulting phenotype provides a model of well-differentiated human thyroid cancer.