Open AccessHarvey-ras oncogene restriction fragment alleles in familial melanoma kindreds
Author(s) -
Colin J. Sutherland,
Helen M. Shaw,
CL Roberts,
Julienne Grace,
M. Stewart,
William H. McCarthy,
Richard Kefford
Publication year - 1986
Publication title -
british journal of cancer
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1986.241
Subject(s) - genetics , allele , locus (genetics) , biology , restriction fragment length polymorphism , restriction fragment , hpaii , genotype , gene , gene expression , dna methylation
Unique and uncommon BamHI allelic restriction fragments of the Ha-ras locus have been reported in the genomes of patients with cancer and of three affected members of a familial melanoma kindred (Krontiris et al., 1986). Analysis of the BamHI and Msp/HpaII restriction fragments of peripheral blood leucocyte DNA from the members of two families with hereditary melanoma (HM)/familial dysplastic naevus syndrome (DNS) revealed that the only Ha-ras allele common to four affected members of one kindred and two from a second kindred, was the 6.7kb allele which is found in 66% of the normal population. This allele was found equally in affected and non-affected family members, and in one affected case was inherited from an unaffected homozygous parent. It was absent in two affected sisters in a third kindred. In the first kindred the karyotype of all three melanoma sufferers was 46XX 9qh+, while six unaffected members had a normal karyotype. BamHI polymorphism of the Ha-ras gene does not identify the affected members in the HM/DNS families studied.