Open AccessClonogenic cell survival in cryopreserved human tumour cells
Author(s) -
Peter J. Selby,
G. Gordon Steel
Publication year - 1981
Publication title -
british journal of cancer
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.833
H-Index - 236
eISSN - 1532-1827
pISSN - 0007-0920
DOI - 10.1038/bjc.1981.22
Subject(s) - clonogenic assay , radiosensitivity , cryopreservation , biology , melanoma , in vitro , pathology , transplantation , cyclophosphamide , cancer research , radiation therapy , medicine , chemotherapy , microbiology and biotechnology , embryo , biochemistry , genetics
Cells from 3 human tumours have been grown in soft agar contained in Millipore diffusion chambers and implanted i.p. in mice. Clonal growth was obtained from fresh biopsy samples, from cryopreserved tissue, and from xenografts of the tissues in immune-suppressed mice. The radiosensitivities of a melanoma and an ovarian carcinoma were evaluated by in vitro irradiation before assay for colony formation. Xenografting did not modify the radiosensitivity of the melanoma. Cells from another tumour were exposed to Adriamycin or cyclophosphamide whilst contained within i.p. diffusion chambers; the sensitivity was similar for cryopreserved and xenografted cells. The results encourage further attempts to quantify the sensitivity of human tumour cells by these methods.