Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts | Zendy

Régis Tournebize | Zendy; А. В. Попов | Zendy; Kazuhisa Kinoshita | Zendy; A J Ashford | Zendy; Sonja Rybina | Zendy; Andrei Pozniakovsky | Zendy; Thomas U. Mayer | Zendy; Claire Walczak | Zendy; Eric Karsenti | Zendy; Anthony A. Hyman | Zendy

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Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

Author(s) -

Régis Tournebize,

А. В. Попов,

Kazuhisa Kinoshita,

A J Ashford,

Sonja Rybina,

Andrei Pozniakovsky,

Thomas U. Mayer,

Claire Walczak,

Eric Karsenti,

Anthony A. Hyman

Publication year - 1999

Publication title -

nature cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 11.38

H-Index - 369

eISSN - 1476-4679

pISSN - 1465-7392

DOI - 10.1038/71330

Subject(s) - xenopus , microtubule , microbiology and biotechnology , microtubule associated protein , biology , microtubule nucleation , cell , genetics , cell cycle , centrosome , gene

Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

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