Open Access‘VIT1’, A Novel Gene Associated with Vitiligo
Author(s) -
Le Poole I. Caroline,
Sarangarajan Rangaprasad,
Zhao Yang,
Stennett Lawrence S.,
Brown Thomas L.,
Sheth Pranav,
Miki Toru,
Boissy Raymond E.
Publication year - 2001
Publication title -
pigment cell research
Language(s) - English
Resource type - Journals
eISSN - 1600-0749
pISSN - 0893-5785
DOI - 10.1034/j.1600-0749.2001.140608.x
Subject(s) - complementary dna , biology , gene , genetics , open reading frame , cdna library , homology (biology) , differential display , gene expression , vitiligo , rna , melanocyte , microbiology and biotechnology , peptide sequence , melanoma
To define genes associated with the pigmentary disorder vitiligo, gene expression was compared in non‐lesional melanocytes cultured from three vitiligo patients and from three control melanocyte cultures by differential display. A basic local alignment search tool search did not reveal homology of six differentially expressed cDNA fragments to previously identified expressed sequence tags; thus, one was used to screen a melanocyte cDNA library. The underlying VIT1 gene maps to chromosome 2p16. The 3′ portion of the VIT1 message is complementary to the 3′ end of hMSH6 mRNA, enabling the formation of RNA–RNA hybrids, which may interfere with G/T mismatch repair function. Moreover, the aligned cDNA sequence revealed an open reading frame identical to a hypothetical protein expressed in brain, with a similarity to Drosophila calmodulin, and containing a zinc‐finger motif partially identical to N ‐recognin. Expression of ORF mRNA was confirmed for multiple skin cell types, suggesting its importance for skin physiology.