Open AccessIncreased MCP‐1 and MIP‐1β in bronchoalveolar lavage fluid of chronic bronchitics
Author(s) -
Capelli A.,
Di Stefano A.,
Gnemmi I.,
Balbo P,
Cerutti C.G.,
Balbi B.,
Lusuardi M.,
Donner C.F.
Publication year - 1999
Publication title -
european respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.021
H-Index - 241
eISSN - 1399-3003
pISSN - 0903-1936
DOI - 10.1034/j.1399-3003.1999.14a27.x
Subject(s) - bronchoalveolar lavage , chronic bronchitis , medicine , gastroenterology , macrophage inflammatory protein , bronchitis , immunology , monocyte , chemokine , inflammation , lung
CC‐chemokines are chemotactic factors expressed in a wide range of cell types and tissues. The aim of this study was to evaluate the involvement of CC‐chemokines in the airways inflammation of patients affected by chronic bronchitis. The study evaluated, with an immunoassay, the concentrations of monocyte chemotactic protein‐1 (MCP‐1), macrophage inflammatory protein‐1α (MIP‐1α) and macrophage inflammatory protein‐1β (MIP‐1β), in the bronchoalveolar lavage fluid (BALF) of 12 smokers affected by chronic bronchitis and 14 smoking, 15 nonsmoking and six exsmoking healthy subjects. MCP‐1 was significantly increased in patients with chronic bronchitis ((mean± sd ) 10.75±4.04 pg·mL ‐1 ) and in the smoker control group (12.39±5.87 pg·mL ‐1 ) compared with healthy exsmokers: (7.12±1.60 pg·mL ‐1 , p=0.035 and p=0.045, respectively) and nonsmokers (6.41±3.87 pg·mL ‐1 , p=0.003 and p=0.006, respectively). MIP‐1α concentrations were undetectable. A significant difference was observed in MIP‐1‐β levels in BALF of chronic bronchitics (8.11±5.97 pg·mL ‐1 ) compared to smoker (3.57±2.90 pg·mL ‐1 , p=0.018), exsmoker (3.43±0.68 pg·mL ‐1 , p=0.025) and nonsmoker (3.39±3.73 pg·mL ‐1 , p=0.008) control groups. A negative correlation was observed between MIP‐1β levels and forced expiratory volume in one second values (ρ= ‐0.64, p=0.035) in chronic bronchitics. An increase of monocyte chemotactic protein‐1 is related to smoking habit and seems consistent with a lung inflammatory reaction. On the contrary, an increase in macrophage inflammatory protein‐1β levels is restricted to smokers developing chronic obstructive pulmonary disease. These data suggest a role of CC‐chemokines in the pathogenesis of chronic bronchitis.