Open AccessOzone‐induced lung function decrements do not correlate with early airway inflammatory or antioxidant responses
Author(s) -
Blomberg A,
Mudway I.s,
Nordenhäll C,
Hedenström H,
Kelly F.j,
Frew A.j,
Holgate S.t,
Sandström T
Publication year - 1999
Publication title -
european respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.021
H-Index - 241
eISSN - 1399-3003
pISSN - 0903-1936
DOI - 10.1034/j.1399-3003.1999.13f31.x
Subject(s) - lung function , antioxidant , airway , ozone , lung , medicine , airway resistance , immunology , biology , chemistry , anesthesia , biochemistry , organic chemistry
This study sought to clarify the early events occurring within the airways of healthy human subjects performing moderate intermittent exercise following ozone challenge. Thirteen healthy nonsmoking subjects were exposed in a single blinded, crossover control fashion to 0.2 parts per million (ppm) O 3 and filtered air for 2 h, using a standard intermittent exercise and rest protocol. Lung function was assessed pre‐ and immediately post‐exposure. Bronchoscopy was performed with endobronchial mucosal biopsies, bronchial wash (BW) and bronchoalveolar lavage (BAL) 1.5 h after the end of the exposure period. Respiratory tract lining fluid (RTLF) redox status was assessed by measuring a range of antioxidants and oxidative damage markers in BW and BAL fluid samples. There was a significant upregulation after O 3 exposure in the expression of vascular endothelial P‐selectin (p<0.005) and intercellular adhesion molecule‐1 (p<0.005). This was associated with a 2‐fold increase in submucosal mast cells (p<0.005) in biopsy samples, without evidence of neutrophilic inflammation, and a decrease in BAL fluid macrophage numbers (1.6‐fold, p<0.005), with an activation of the remaining macrophage subset (2.5‐fold increase in % human leukocyte antigen (HLA)‐DR+ cells, p<0.005). In addition, exposure led to a 4.5‐fold and 3.1‐fold increase of reduced glutathione (GSH) concentrations, in BW and BAL fluid respectively (p<0.05), with alterations in urate and α‐tocopherol plasma/RTLF partitioning ratios (p<0.05). Spirometry showed reductions in forced vital capacity (p<0.05) and forced expiratory volume in one second (p<0.01), with evidence of small airway narrowing using forced expiratory flow values (p<0.005). Evidence was found of O 3 ‐induced early adhesion molecule upregulation, increased submucosal mast cell numbers and alterations to the respiratory tract lining fluid redox status. No clear relationship was demonstrable between changes in these early markers and the lung function decrements observed. The results therefore indicate that the initial lung function decrements are not predictive of, or causally related to the O 3 ‐induced inflammatory events in normal human subjects.