Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue | Zendy

Jiao Ma | Zendy; Carl Ward | Zendy; Irwin Jungreis | Zendy; Sarah A. Slavoff | Zendy; Adam G. Schwaid | Zendy; John M. Neveu | Zendy; Bogdan Budnik | Zendy; Manolis Kellis | Zendy; Alan Saghatelian | Zendy

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Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue

Author(s) -

Jiao Ma,

Carl Ward,

Irwin Jungreis,

Sarah A. Slavoff,

Adam G. Schwaid,

John M. Neveu,

Bogdan Budnik,

Manolis Kellis,

Alan Saghatelian

Publication year - 2014

Publication title -

journal of proteome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.644

H-Index - 161

eISSN - 1535-3907

pISSN - 1535-3893

DOI - 10.1021/pr401280w

Subject(s) - biology , human proteome project , proteome , human genome , computational biology , genome , proteogenomics , open reading frame , proteomics , translation (biology) , human cell , k562 cells , gene , genetics , genomics , peptide sequence , messenger rna

The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases the size of the genome and the proteome and provides insights into the molecular biology of mammalian cells, such as the prevalent usage of non-AUG start codons. Through modifications of the existing SEP-discovery workflow, we discover an additional 195 SEPs in K562 cells and extend this methodology to identify novel human SEPs in additional cell lines and human tissue for a final tally of 237 new SEPs. These results continue to expand the human genome and proteome and demonstrate that SEPs are a ubiquitous class of nonannotated polypeptides that require further investigation.

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