Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli | Zendy

Dimitri Dascier | Zendy; Spiros Kambourakis | Zendy; Ling Hua | Zendy; J. David Rozzell | Zendy; Jon D. Stewart | Zendy

Open Access

Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli

Author(s) -

Dimitri Dascier,

Spiros Kambourakis,

Ling Hua,

J. David Rozzell,

Jon D. Stewart

Publication year - 2014

Publication title -

organic process research and development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.904

H-Index - 109

eISSN - 1520-586X

pISSN - 1083-6160

DOI - 10.1021/op400312n

Subject(s) - acetophenone , cofactor , ketone , chemistry , nicotinamide , nad+ kinase , enzyme , regeneration (biology) , dehydrogenase , biocatalysis , combinatorial chemistry , catalysis , organic chemistry , reaction mechanism , biology , microbiology and biotechnology

This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a bis -trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully.

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