A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways | Zendy

Shanteri Singh | Zendy; Pauline PeltierPain | Zendy; Marco Tonelli | Zendy; Jon S. Thorson | Zendy

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A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways

Author(s) -

Shanteri Singh,

Pauline PeltierPain,

Marco Tonelli,

Jon S. Thorson

Publication year - 2014

Publication title -

organic letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.94

H-Index - 239

eISSN - 1523-7060

pISSN - 1523-7052

DOI - 10.1021/ol501241a

Subject(s) - chemistry , in situ , sugar , characterization (materials science) , nucleotide , biochemistry , stereochemistry , organic chemistry , nanotechnology , gene , materials science

A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

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