Open AccessAntileishmanial Metabolites from Geosmithia langdonii
Author(s) -
Lourin G. Malak,
Mohamed A. Ibrahim,
D. Bishay,
Afaf M. Abdel-Baky,
A. M. Moharram,
Babu L. Tekwani,
Stephen J. Cutler,
Samir A. Ross
Publication year - 2014
Publication title -
journal of natural products
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.976
H-Index - 139
eISSN - 1520-6025
pISSN - 0163-3864
DOI - 10.1021/np5000473
Subject(s) - leishmania donovani , fractionation , stereochemistry , two dimensional nuclear magnetic resonance spectroscopy , absolute configuration , chemistry , leishmania , leishmania major , bioassay , biology , chromatography , parasite hosting , world wide web , computer science , genetics
Antileishmanial bioassay guided fractionation of Geosmithia langdonii has resulted in the isolation and identification of two new compounds (1 and 2) together with 10 known compounds (3-12). The structures of the isolated metabolites were elucidated based on comprehensive 1D and 2D NMR spectroscopic data as well as mass spectrometry. The absolute configuration at C4, C5, and C6 of 2 was determined as R using a modified Mosher esterification method and NOESY correlations. The extracts and the isolated metabolites were evaluated for their antileishmanial activities. Compounds 3, 9, 11, and 12 were found to be active against Leishmania donovani with IC50 values of 6.9, 3.3, 8.5, and 9.2 μM, respectively, while compounds 1, 5, and 10 showed lower activities against L. donovani with IC50 values of 13.0, 47.3, and 34.0 μM, respectively.
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