DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH | Zendy

Giovanni Maglia | Zendy; Marsiyana M. Henricus | Zendy; Romain Wyss | Zendy; Qiuhong Li | Zendy; Stephen Cheley | Zendy; Hagan Bayley | Zendy

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DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH

Author(s) -

Giovanni Maglia,

Marsiyana M. Henricus,

Romain Wyss,

Qiuhong Li,

Stephen Cheley,

Hagan Bayley

Publication year - 2009

Publication title -

nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.853

H-Index - 488

eISSN - 1530-6992

pISSN - 1530-6984

DOI - 10.1021/nl9020232

Subject(s) - nanopore , nucleic acid , dna , analyte , chemistry , molecule , nanopore sequencing , biomolecule , nanotechnology , biophysics , dna sequencing , biochemistry , materials science , biology , chromatography , organic chemistry

Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the development of systems for rapid, low-cost DNA sequencing. Here, we show that DNA can be translocated through an engineered alphaHL protein pore at pH 11.7, a value at which dsDNA is denatured. Therefore, the alphaHL pore is sufficiently stable to entertain the possibility of direct nanopore sequencing of genomic dsDNA samples, which are more readily obtained and handled than ssDNA.

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