Highly Parallel Transport Recordings on a Membrane-on-Nanopore Chip at Single Molecule Resolution | Zendy

Mi­chael Urban | Zendy; Alexander Kleefen | Zendy; Nobina Mukherjee | Zendy; Patrick Seelheim | Zendy; Barbara Windschiegl | Zendy; Marc Vor der Brüggen | Zendy; Armağan Koçer | Zendy; Robert Tampé | Zendy

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Highly Parallel Transport Recordings on a Membrane-on-Nanopore Chip at Single Molecule Resolution

Author(s) -

Michael Urban,

Alexander Kleefen,

Nobina Mukherjee,

Patrick Seelheim,

Barbara Windschiegl,

Marc Vor der Brüggen,

Armağan Koçer,

Robert Tampé

Publication year - 2014

Publication title -

nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.853

H-Index - 488

eISSN - 1530-6992

pISSN - 1530-6984

DOI - 10.1021/nl5002873

Subject(s) - nanopore , membrane , nanotechnology , chemistry , ion channel , resolution (logic) , biophysics , materials science , biochemistry , computer science , receptor , biology , artificial intelligence

Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250,000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

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