Supported Membranes Embedded with Fixed Arrays of Gold Nanoparticles | Zendy

Theobald Lohmüller | Zendy; Sara B. Triffo | Zendy; Geoff P. O’Donoghue | Zendy; Qian Xu | Zendy; Michael P. Coyle | Zendy; Jay T. Groves | Zendy

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Supported Membranes Embedded with Fixed Arrays of Gold Nanoparticles

Author(s) -

Theobald Lohmüller,

Sara B. Triffo,

Geoff P. O’Donoghue,

Qian Xu,

Michael P. Coyle,

Jay T. Groves

Publication year - 2011

Publication title -

nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.853

H-Index - 488

eISSN - 1530-6992

pISSN - 1530-6984

DOI - 10.1021/nl202847t

Subject(s) - membrane , nanoparticle , materials science , colloidal gold , juxtacrine signalling , lipid bilayer , soft lithography , substrate (aquarium) , nanotechnology , monolayer , self assembly , chemical engineering , chemistry , biochemistry , medicine , receptor , alternative medicine , oceanography , pathology , fabrication , geology , engineering , paracrine signalling

We present a supported membrane platform consisting of a fluid lipid bilayer membrane embedded with a fixed array of gold nanoparticles. The system is realized by preforming a hexagonal array of gold nanoparticles (∼5-7 nm) with controlled spacing (∼50-150 nm) fixed to a silica or glass substrate by block copolymer lithography. Subsequently, a supported membrane is assembled over the intervening bare substrate. Proteins or other ligands can be associated with the fluid lipid component, the fixed nanoparticle component, or both, providing a hybrid interface consisting of mobile and immobile components with controlled geometry. We test different biochemical coupling strategies to bind individual proteins to the particles surrounded by a fluid lipid membrane. The coupling efficiency to nanoparticles and the influence of nanoparticle arrays on the surrounding membrane integrity are characterized by fluorescence imaging, correlation spectroscopy, and super-resolution fluorescence microscopy. Finally, the functionality of this system for live cell experiments is tested using the ephrin-A1-EphA2 juxtacrine signaling interaction in human breast epithelial cells.

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