Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy | Zendy

Diana B. Peckys | Zendy; Niels de Jonge | Zendy

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Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy

Author(s) -

Diana B. Peckys,

Niels de Jonge

Publication year - 2011

Publication title -

nano letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.853

H-Index - 488

eISSN - 1530-6992

pISSN - 1530-6984

DOI - 10.1021/nl200285r

Subject(s) - vesicle , transmission electron microscopy , colloidal gold , micrometer , scanning electron microscope , nanoparticle , nanotechnology , nanoscopic scale , materials science , biophysics , microscopy , membrane , intracellular , chemistry , analytical chemistry (journal) , chromatography , optics , biology , biochemistry , physics , composite material

The intracellular uptake of 30 nm diameter gold nanoparticles (Au-NPs) was studied at the nanoscale in pristine eukaryotic cells. Live COS-7 cells were maintained in a microfluidic chamber and imaged using scanning transmission electron microscopy. A quantitative image analysis showed that Au-NPs bound to the membranes of vesicles, possibly lysosomes, and occupied 67% of the available surface area. The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles.

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