Reversible, Meniscus-Free Molecular Combing of Long-Chain DNA | Zendy

J. Koota | Zendy; Ina Seuffert | Zendy; H. Li | Zendy; Roman Lehner | Zendy; Thomas Gisler | Zendy

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Reversible, Meniscus-Free Molecular Combing of Long-Chain DNA

Author(s) -

J. Koota,

Ina Seuffert,

H. Li,

Roman Lehner,

Thomas Gisler

Publication year - 2007

Publication title -

langmuir

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.042

H-Index - 333

eISSN - 1520-5827

pISSN - 0743-7463

DOI - 10.1021/la7013122

Subject(s) - combing , meniscus , dna , desorption , chemistry , adsorption , deposition (geology) , chemical engineering , nanotechnology , materials science , organic chemistry , biochemistry , optics , composite material , paleontology , physics , incidence (geometry) , sediment , biology , engineering

We introduce a method for reversibly orienting long-chain DNA on solid hydrophilic substrates without a fluid meniscus. End-tethered lambda-DNA mushrooms are elongated by a hydrodynamic flow in the presence of trivalent cations, resulting in electrostatic adsorption of the extended DNA to the surface. By complexation of the cations the part of the DNA which is unspecifically bound to the surface desorbs quantitatively, and the mushroom conformation is restored. With the use of multiple deposition-combing steps, combined with a final desorption step, tethering densities higher than attainable with single deposition steps can be obtained.

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