Open AccessIon Channel Probes for Scanning Ion Conductance Microscopy
Author(s) -
Yi Zhou,
Leonard K. Bright,
Wenqing Shi,
Craig A. Aspinwall,
Lane A. Baker
Publication year - 2014
Publication title -
langmuir
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.042
H-Index - 333
eISSN - 1520-5827
pISSN - 0743-7463
DOI - 10.1021/la504097f
Subject(s) - ion channel , scanning ion conductance microscopy , conductance , ion , ion current , chemistry , chemical imaging , nanotechnology , lipid bilayer , microscope , resolution (logic) , microscopy , membrane , analytical chemistry (journal) , biophysics , materials science , scanning confocal electron microscopy , optics , chromatography , biochemistry , computer science , physics , receptor , organic chemistry , artificial intelligence , hyperspectral imaging , condensed matter physics , biology
The sensitivity and selectivity of ion channels provide an appealing opportunity for sensor development. Here, we describe ion channel probes (ICPs), which consist of multiple ion channels reconstituted into lipid bilayers suspended across the opening of perflourinated glass micropipets. When incorporated with a scanning ion conductance microscope (SICM), ICPs displayed a distance-dependent current response that depended on the number of ion channels in the membrane. With distance-dependent current as feedback, probes were translated laterally, to demonstrate the possibility of imaging with ICPs. The ICP platform yields several potential advantages for SICM that will enable exciting opportunities for incorporation of chemical information into imaging and for high-resolution imaging.
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