Open AccessSensing Reversible Protein–Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors
Author(s) -
A. Münzer,
Wanji Seo,
Gregory J. Morgan,
Zachary P. Michael,
Yong Zhao,
Katharina Melzer,
Giuseppe Scarpa,
Alexander Star
Publication year - 2014
Publication title -
the journal of physical chemistry c
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 289
eISSN - 1932-7455
pISSN - 1932-7447
DOI - 10.1021/jp503670a
Subject(s) - carbon nanotube , linker , materials science , streptavidin , field effect transistor , nanotechnology , raman spectroscopy , avidin , biotin , chemistry , transistor , biochemistry , physics , quantum mechanics , voltage , computer science , optics , operating system
We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker. Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed. Furthermore, protein "fingerprints" of NeutrAvidin and streptavidin were obtained by monitoring their adsorption at several pH values. Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom