Open AccessHigh Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals
Author(s) -
Matthew K. Kiesewetter,
Vladimir K. Michaelis,
Joseph J. Walish,
Robert G. Griffin,
Timothy M. Swager
Publication year - 2014
Publication title -
the journal of physical chemistry b
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 392
eISSN - 1520-6106
pISSN - 1520-5207
DOI - 10.1021/jp410387e
Subject(s) - chemistry , magnetic dipole–dipole interaction , pulmonary surfactant , nuclear overhauser effect , aqueous solution , nuclear magnetic resonance spectroscopy , residual dipolar coupling , paramagnetism , chemical physics , polarization (electrochemistry) , relaxation (psychology) , nuclear magnetic resonance , dipole , organic chemistry , psychology , social psychology , biochemistry , physics , quantum mechanics
We illustrate the ability to place a water-insoluble biradical, bTbk, into a glycerol/water matrix with the assistance of a surfactant, sodium octyl sulfate (SOS). This surfactant approach enables a previously water insoluble biradical, bTbk, with favorable electron-electron dipolar coupling to be used for dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) experiments in frozen, glassy, aqueous media. Nuclear Overhauser enhancement (NOE) and paramagnetic relaxation enhancement (PRE) experiments are conducted to determine the distribution of urea and several biradicals within the SOS macromolecular assembly. We also demonstrate that SOS assemblies are an effective approach by which mixed biradicals are created through an assembly process.
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