Bio-functionalization of Monodisperse Magnetic Nanoparticles and Their Use as Biomolecular Labels in a Magnetic Tunnel Junction Based Sensor | Zendy

Stephanie G. Grancharov | Zendy; Hao Zeng | Zendy; Shouheng Sun | Zendy; Shan X. Wang | Zendy; Stephen O’Brien | Zendy; Christopher B. Murray | Zendy; J. R. Kirtley | Zendy; G. A. Held | Zendy

Open Access

Bio-functionalization of Monodisperse Magnetic Nanoparticles and Their Use as Biomolecular Labels in a Magnetic Tunnel Junction Based Sensor

Author(s) -

Stephanie G. Grancharov,

Hao Zeng,

Shouheng Sun,

Shan X. Wang,

Stephen O’Brien,

Christopher B. Murray,

J. R. Kirtley,

G. A. Held

Publication year - 2005

Publication title -

the journal of physical chemistry b

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.864

H-Index - 392

eISSN - 1520-6106

pISSN - 1520-5207

DOI - 10.1021/jp051098c

Subject(s) - surface modification , biosensor , magnetic nanoparticles , nanotechnology , materials science , dispersity , nanoparticle , superparamagnetism , iron oxide nanoparticles , silicon , chemical engineering , optoelectronics , magnetic field , magnetization , polymer chemistry , engineering , physics , quantum mechanics

Monodisperse magnetic nanoparticles (NPs) could enable the ultra-sensitive magnetic detection of biological analytes. However, rendering these particles biocompatible has remained a challenge. We report the bio-functionalization and detection of 12-nm manganese ferrite NPs. We have achieved the site-specific binding of biotin-functionalized NPs onto avidin-patterned silicon oxide substrates and DNA-functionalized NPs onto complementary DNA-patterned silicon oxide substrates. Utilizing scanning SQUID microscopy, we show that these substrate-bound NPs retain their magnetic properties. Finally, we demonstrate a novel method of detecting either protein binding or DNA hybridization at room temperature using the NPs and a magnetic tunnel-junction-based biosensor situated in orthogonal magnetic fields.

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