Sequential Injection Lab-on-Valve System for the Determination of the Activity of Peroxidase in Vegetables | Zendy

Susana S. M. P. Vidigal | Zendy; Ildikó V. Tóth | Zendy; António O.S.S. Rangel | Zendy

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Sequential Injection Lab-on-Valve System for the Determination of the Activity of Peroxidase in Vegetables

Author(s) -

Susana S. M. P. Vidigal,

Ildikó V. Tóth,

António O.S.S. Rangel

Publication year - 2010

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/jf9035113

Subject(s) - abts , peroxidase , hydrogen peroxide , chemistry , horseradish peroxidase , chromatography , flow injection analysis , enzyme , biochemistry , antioxidant , detection limit , dpph

Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometric determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H(2)O(2)) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15 microL per assay and a consumption of ABTS and H(2)O(2) of 24 microg and 12 microg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.

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