Development of a Multianalyte Enzyme-Linked Immunosorbent Assay for Permethrin and Aroclors and Its Implementation for Analysis of Soil/Sediment and House Dust Extracts | Zendy

Alisa Bronshtein | Zendy; Jane C. Chuang | Zendy; Jeanette M. Van Emon | Zendy; Miriam Altstein | Zendy

Open Access

Development of a Multianalyte Enzyme-Linked Immunosorbent Assay for Permethrin and Aroclors and Its Implementation for Analysis of Soil/Sediment and House Dust Extracts

Author(s) -

Alisa Bronshtein,

Jane C. Chuang,

Jeanette M. Van Emon,

Miriam Altstein

Publication year - 2012

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/jf300043g

Subject(s) - permethrin , analyte , chromatography , chemistry , immunoassay , sediment , matrix (chemical analysis) , detection limit , environmental chemistry , pesticide , antibody , paleontology , agronomy , biology , immunology

Development of a multianalyte enzyme-linked immunosorbent assay (ELISA) for detection of permethrin and aroclors 1248 or 1254 and implementation of the assay for analysis of soil/sediment samples are described. The feasibility of using the multianalyte ELISA to monitor aroclors 1254 and permethrin simultaneously was tested with permethrin and aroclor standards and with aroclor- and permethrin-containing soil/sediment and house dust samples. Comparison of the I₅₀ and I₂₀ values of the multianalyte with those of a single-analyte assay revealed similar results, and multianalyte ELISA determination of analyte amounts in soil/sediment dust samples yielded similar results to those of a single-analyte assay. A single-analyte assay of permethrin content in permethrin-containing dust samples showed that the ELISA can determine the analyte accurately in samples with dust matrix contents ranging from 6.25 to 100 mg as indicated by the good correlation between the results of the immunoassay and those of the gas chromatography analysis.

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