Event-Specific Real-Time Detection and Quantification of Genetically Modified Roundup Ready Soybean | Zendy

Huang Chia-Chia | Zendy; TzuMing Pan | Zendy

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Event-Specific Real-Time Detection and Quantification of Genetically Modified Roundup Ready Soybean

Author(s) -

Huang Chia-Chia,

TzuMing Pan

Publication year - 2005

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/jf048580x

Subject(s) - primer (cosmetics) , genetically modified organism , detection limit , primer extension , real time polymerase chain reaction , biology , genomic dna , computational biology , european union , dna , gene , genetics , chromatography , chemistry , base sequence , organic chemistry , business , economic policy

The event-specific real-time detection and quantification of Roundup Ready soybean (RRS) using an ABI PRISM 7700 sequence detection system with light upon extension (LUX) primer was developed in this study. The event-specific primers were designed, targeting the junction of the RRS 5' integration site and the endogenous gene lectin1. Then, a standard reference plasmid was constructed that carried both of the targeted sequences for quantitative analysis. The detection limit of the LUX real-time PCR system was 0.05 ng of 100% RRS genomic DNA, which was equal to 20.5 copies. The range of quantification was from 0.1 to 100%. The sensitivity and range of quantification successfully met the requirement of the labeling rules in the European Union and Taiwan.

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