Open Access2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
Author(s) -
Marius M. Haugland,
Afaf H. ElSagheer,
Rachel J. Porter,
Javier Peña,
Tom Brown,
Edward A. Anderson,
Janet E. Lovett
Publication year - 2016
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/jacs.6b05421
Subject(s) - chemistry , electron paramagnetic resonance , site directed spin labeling , electron paramagnetic resonance spectroscopy , spectroscopy , spin label , dna , nuclear magnetic resonance spectroscopy , azide , duplex (building) , cycloaddition , crystallography , stereochemistry , nuclear magnetic resonance , catalysis , organic chemistry , biochemistry , physics , quantum mechanics
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
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