Reversible Energy-Transfer Switching on a DNA Scaffold | Zendy

Magnus Bälter | Zendy; Martin Hammarson | Zendy; Patricia Remón | Zendy; Shiming Li | Zendy; Nittaya Gale | Zendy; Tom Brown | Zendy; Joakim Andréasson | Zendy

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Reversible Energy-Transfer Switching on a DNA Scaffold

Author(s) -

Magnus Bälter,

Martin Hammarson,

Patricia Remón,

Shiming Li,

Nittaya Gale,

Tom Brown,

Joakim Andréasson

Publication year - 2015

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja512416n

Subject(s) - spiropyran , merocyanine , chemistry , förster resonance energy transfer , isomerization , photochromism , photochemistry , quenching (fluorescence) , energy transfer , derivative (finance) , fluorescence , covalent bond , excited state , scaffold , biophysics , dna , chemical physics , catalysis , biochemistry , organic chemistry , medicine , physics , quantum mechanics , biology , nuclear physics , financial economics , biomedical engineering , economics

We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

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