A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO) | Zendy

Alexandra T. Wrobel | Zendy; Timothy C. Johnstone | Zendy; Alexandria Deliz Liang | Zendy; Stephen J. Lippard | Zendy; Pablo RiveraFuentes | Zendy

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A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)

Author(s) -

Alexandra T. Wrobel,

Timothy C. Johnstone,

Alexandria Deliz Liang,

Stephen J. Lippard,

Pablo RiveraFuentes

Publication year - 2014

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja500315x

Subject(s) - chemistry , nitroxyl , fluorescence , fluorophore , photobleaching , photochemistry , zinc , infrared , inorganic chemistry , organic chemistry , physics , quantum mechanics , optics

The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.

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