The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment | Zendy

Catherine L. Grimes | Zendy; Lushanti De Zoysa Ariyananda | Zendy; James E. Melnyk | Zendy; Erin K. O’Shea | Zendy

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The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment

Author(s) -

Catherine L. Grimes,

Lushanti De Zoysa Ariyananda,

James E. Melnyk,

Erin K. O’Shea

Publication year - 2012

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja303883c

Subject(s) - chemistry , innate immune system , nod2 , fragment (logic) , bacterial cell structure , immune system , innate lymphoid cell , microbiology and biotechnology , biochemistry , bacteria , immunology , receptor , genetics , computer science , programming language , biology

Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn's disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP.

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