Fluorescence of Natural DNA: From the Femtosecond to the Nanosecond Time Scales | Zendy

Ignacio Vayá | Zendy; T. Gustavsson | Zendy; FrançoisAlexandre Miannay | Zendy; Thierry Douki | Zendy; Dimitra Markovitsi | Zendy

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Fluorescence of Natural DNA: From the Femtosecond to the Nanosecond Time Scales

Author(s) -

Ignacio Vayá,

T. Gustavsson,

FrançoisAlexandre Miannay,

Thierry Douki,

Dimitra Markovitsi

Publication year - 2010

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja102800r

Subject(s) - chemistry , fluorescence , nanosecond , chromophore , femtosecond , photon upconversion , excimer , fluorescence spectroscopy , time resolved spectroscopy , resonance fluorescence , fluorescence cross correlation spectroscopy , laser induced fluorescence , spectroscopy , dna , photochemistry , molecular physics , optics , laser , physics , ion , biochemistry , organic chemistry , quantum mechanics

The fluorescence of calf thymus DNA is studied by steady-state and time-resolved spectroscopy combining fluorescence upconversion and time-correlated single photon counting. The fluorescence spectrum is very similar to that of a stoichiometric mixture of monomeric chromophores, arising from bright pi pi* states, and contrasts with the existing picture of exciplex emission in natural DNA. Yet, the DNA fluorescence decays span over five decades of time, with 98% of the photons being emitted at times longer than 10 ps. These findings, in association with recent studies on model duplexes, are explained by the involvement of dark states, possibly related to charge separation, serving as a reservoir for the repopulation of the bright pi pi* states.

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