DNA−Gold Nanorod Conjugates for Remote Control of Localized Gene Expression by near Infrared Irradiation | Zendy

ChiaChun Chen | Zendy; Yen-Ping Lin | Zendy; Chih-Wei Wang | Zendy; Hsiao-Chien Tzeng | Zendy; Chia-Hsuan Wu | Zendy; Yi-Cheng Chen | Zendy; Chin-Pei Chen | Zendy; LiChyong Chen | Zendy; YiChun Wu | Zendy

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DNA−Gold Nanorod Conjugates for Remote Control of Localized Gene Expression by near Infrared Irradiation

Author(s) -

ChiaChun Chen,

Yen-Ping Lin,

Chih-Wei Wang,

Hsiao-Chien Tzeng,

Chia-Hsuan Wu,

Yi-Cheng Chen,

Chin-Pei Chen,

LiChyong Chen,

YiChun Wu

Publication year - 2006

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja0570180

Subject(s) - nanorod , chemistry , hela , irradiation , green fluorescent protein , biophysics , fluorescence , gene expression , dna , microbiology and biotechnology , gene , nanotechnology , in vitro , biochemistry , materials science , optics , biology , physics , nuclear physics

Gold nanorods were attached to the gene of enhanced green fluorescence protein (EGFP) for the remote control of gene expression in living cells. The UV-vis spectroscopy, electrophoresis, and transmission electron microscopy (TEM) were used to study the optical and structural properties of the EGFP DNA and gold nanorod (EGFP-GNR) conjugates before and after femto-second near-infrared (NIR) laser irradiation. Upon NIR irradiation, the gold nanorods of EGFP-GNR conjugates underwent shape transformation that resulted in the release of EGFP DNA. When EGFP-GNR conjugates were delivered to cultured HeLa cells, induced GFP expression was specifically observed in cells that were locally exposed to NIR irradiation. Our results demonstrate the feasibility of using gold nanorods and NIR irradiation as means of remote control of gene expression in specific cells. This approach has potential applications in biological and medical studies.

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