A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP | Zendy

Peter Sazani | Zendy; Rosa Larralde | Zendy; Jack W. Szostak | Zendy

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A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP

Author(s) -

Peter Sazani,

Rosa Larralde,

Jack W. Szostak

Publication year - 2004

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja049171k

Subject(s) - aptamer , chemistry , adenosine triphosphate , phosphorylation , rna , molecular recognition , nucleotide , biochemistry , in vitro , biophysics , molecule , microbiology and biotechnology , gene , organic chemistry , biology

We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5' phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5' position. By applying a selective pressure that demands recognition of the 5' triphosphate, we obtained an aptamer that binds to ATP with a Kd of approximately 5 muM, and to AMP with a Kd of approximately 5.5 mM, a difference of 1100-fold. This aptamer demonstrates the ability of small RNAs to interact with negatively charged moieties.

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