Screening of Protein Crystallization Conditions on a Microfluidic Chip Using Nanoliter-Size Droplets | Zendy

Bo Zheng | Zendy; L. Spencer Roach | Zendy; Rustem F. Ismagilov | Zendy

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Screening of Protein Crystallization Conditions on a Microfluidic Chip Using Nanoliter-Size Droplets

Author(s) -

Bo Zheng,

L. Spencer Roach,

Rustem F. Ismagilov

Publication year - 2003

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja037166v

Subject(s) - crystallization , chemistry , protein crystallization , microfluidics , bottleneck , mixing (physics) , microfluidic chip , chromatography , chemical engineering , nanotechnology , organic chemistry , materials science , physics , engineering , quantum mechanics , computer science , embedded system

Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.

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