Selective Binding of Mannose-Encapsulated Gold Nanoparticles to Type 1 Pili in Escherichia coli | Zendy

ChunCheng Lin | Zendy; Yi-Chun Yeh | Zendy; Chan-Yi Yang | Zendy; Chan-Long Chen | Zendy; Gee-Fong Chen | Zendy; ChiaChun Chen | Zendy; YiChun Wu | Zendy

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Selective Binding of Mannose-Encapsulated Gold Nanoparticles to Type 1 Pili in Escherichia coli

Author(s) -

ChunCheng Lin,

Yi-Chun Yeh,

Chan-Yi Yang,

Chan-Long Chen,

Gee-Fong Chen,

ChiaChun Chen,

YiChun Wu

Publication year - 2002

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja0200903

Subject(s) - mannose , chemistry , pilus , colloidal gold , bacterial adhesin , nanoparticle , escherichia coli , biophysics , ligand (biochemistry) , transmission electron microscopy , biochemistry , nanotechnology , receptor , materials science , biology , gene

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

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