Effect of Activated Carbon Amendment on Bacterial Community Structure and Functions in a PAH Impacted Urban Soil | Zendy

Paola Meynet | Zendy; Sarah E. Hale | Zendy; Russell J. Davenport | Zendy; Gerard Cornelissen | Zendy; Gijs D. Breedveld | Zendy; David Werner | Zendy

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Effect of Activated Carbon Amendment on Bacterial Community Structure and Functions in a PAH Impacted Urban Soil

Author(s) -

Paola Meynet,

Sarah E. Hale,

Russell J. Davenport,

Gerard Cornelissen,

Gijs D. Breedveld,

David Werner

Publication year - 2012

Publication title -

environmental science and technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.851

H-Index - 397

eISSN - 1520-5851

pISSN - 0013-936X

DOI - 10.1021/es2043905

Subject(s) - amendment , rhodococcus , environmental chemistry , biodegradation , temperature gradient gel electrophoresis , microbial population biology , chemistry , soil water , environmental remediation , pollutant , soil carbon , bioremediation , contamination , environmental science , bacteria , biology , ecology , 16s ribosomal rna , organic chemistry , soil science , biochemistry , genetics , political science , law , gene , enzyme

We collected urban soil samples impacted by polycyclic aromatic hydrocarbons (PAHs) from a sorbent-based remediation field trial to address concerns about unwanted side-effects of 2% powdered (PAC) or granular (GAC) activated carbon amendment on soil microbiology and pollutant biodegradation. After three years, total microbial cell counts and respiration rates were highest in the GAC amended soil. The predominant bacterial community structure derived from denaturing gradient gel electrophoresis (DGGE) shifted more strongly with time than in response to AC amendment. DGGE band sequencing revealed the presence of taxa with closest affiliations either to known PAH degraders, e.g. Rhodococcus jostii RHA-1, or taxa known to harbor PAH degraders, e.g. Rhodococcus erythropolis, in all soils. Quantification by real-time polymerase chain reaction yielded similar dioxygenases gene copy numbers in unamended, PAC-, or GAC-amended soil. PAH availability assessments in batch tests showed the greatest difference of 75% with and without biocide addition for unamended soil, while the lowest PAH availability overall was measured in PAC-amended, live soil. We conclude that AC had no detrimental effects on soil microbiology, AC-amended soils retained the potential to biodegrade PAHs, but the removal of available pollutants by biodegradation was most notable in unamended soil.

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