Open AccessWrestling with Native Chemical Ligation
Author(s) -
Hagan Bayley,
Stephen Cheley,
Leon Harrington,
Ruhma Syeda
Publication year - 2009
Publication title -
acs chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.899
H-Index - 111
eISSN - 1554-8937
pISSN - 1554-8929
DOI - 10.1021/cb900304p
Subject(s) - semisynthesis , native chemical ligation , ligation , chemistry , chemical ligation , potassium channel , amino acid , membrane , template , biochemistry , computational biology , biophysics , biology , nanotechnology , chemical synthesis , microbiology and biotechnology , in vitro , materials science
An improved method for the semisynthesis of a potassium channel involving native chemical ligation allows the introduction of short sequences containing non-canonical amino acids at any position within the polypeptide chain. The work enhances the technology available for a range of fundamental investigations of membrane proteins and for applications of membrane channels and pores in biotechnology.