New Approaches to In-Cell Detection of Protein Activity: Genetically Encoded Chemiluminescence Probes Pave the Way to Robust HTS Assays | Zendy

Louis Hodgson | Zendy

Open Access

New Approaches to In-Cell Detection of Protein Activity: Genetically Encoded Chemiluminescence Probes Pave the Way to Robust HTS Assays

Author(s) -

Louis Hodgson

Publication year - 2008

Publication title -

acs chemical biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.899

H-Index - 111

eISSN - 1554-8937

pISSN - 1554-8929

DOI - 10.1021/cb800136v

Subject(s) - luciferase , biosensor , chemiluminescence , firefly protocol , computational biology , nanotechnology , computer science , biology , chemistry , biochemistry , genetics , cell culture , materials science , transfection , chromatography , zoology

New genetically encoded biosensors utilizing the modified firefly luciferase promise a great improvement in the signal-to-noise ratio and the dynamic range of response in living cells. These biosensors are particularly suitable for high-throughput screening assays that use large-well-capacity formats because of their excellent response characteristics. The biosensor design strategies are highly generalizable and will be extremely valuable for expanding the repertoire of screenable targets in living cell systems.

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