Open AccessSensing Single Base Incorporation with Nanopore Micromanipulation
Author(s) -
Vincent Croquette
Publication year - 2008
Publication title -
acs chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.899
H-Index - 111
eISSN - 1554-8937
pISSN - 1554-8929
DOI - 10.1021/cb8000205
Subject(s) - nanopore , enzyme , sensitivity (control systems) , nanopore sequencing , dna , biophysics , chemistry , polymerase , computational biology , biological system , nanotechnology , computer science , biology , materials science , biochemistry , dna sequencing , engineering , electronic engineering
Using single-molecule techniques, scientists can routinely investigate the action of a single enzyme. The goal of such studies is usually to gain accurate information unachievable in ensemble assays, such as the maximum instantaneous rate of reaction, the existence of pauses or backward steps, etc. In the article discussed here, the authors have increased their experimental sensitivity so that they can detect a single enzymatic cycle. This improvement makes possible the use of a polymerase enzyme to sequence a single DNA molecule.