First Evidence of a Functional Interaction between DNA Quadruplexes and Poly(ADP-ribose) Polymerase-1 | Zendy

В А Солдатенков | Zendy; Alexandre A. Vetcher | Zendy; Tetyana Duka | Zendy; Sylvain Ladame | Zendy

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First Evidence of a Functional Interaction between DNA Quadruplexes and Poly(ADP-ribose) Polymerase-1

Author(s) -

В А Солдатенков,

Alexandre A. Vetcher,

Tetyana Duka,

Sylvain Ladame

Publication year - 2008

Publication title -

acs chemical biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.899

H-Index - 111

eISSN - 1554-8937

pISSN - 1554-8929

DOI - 10.1021/cb700234f

Subject(s) - telomere , g quadruplex , polymerase , dna , poly adp ribose polymerase , transcription (linguistics) , biochemistry , microbiology and biotechnology , biology , in vitro , chemistry , ribose , enzyme , linguistics , philosophy

We discovered that the abundant human nuclear protein poly(ADP-ribose) polymerase-1 (hPARP-1) binds to intramolecular DNA quadruplexes in vitro with high affinity and with a stoichiometry of two proteins for one quadruplex. Using an enzymatic assay, we have shown that hPARP-1 gets catalytically activated upon binding to G-quadruplexes localized at the c-kit promoter and human telomere regions. This is the first example of a truly functional quadruplex-protein interaction, which has possible implications in understanding hPARP-1 mediated mechanisms of transcription regulation and telomere end protection.

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