Modular Isotopomer Synthesis of γ-Hydroxybutyric Acid for a Quantitative Analysis of Metabolic Fates | Zendy

Sushabhan Sadhukhan | Zendy; GuoFang Zhang | Zendy; Gregory P. Tochtrop | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Modular Isotopomer Synthesis of γ-Hydroxybutyric Acid for a Quantitative Analysis of Metabolic Fates

Author(s) -

Sushabhan Sadhukhan,

GuoFang Zhang,

Gregory P. Tochtrop

Publication year - 2014

Publication title -

acs chemical biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.899

H-Index - 111

eISSN - 1554-8937

pISSN - 1554-8929

DOI - 10.1021/cb500380b

Subject(s) - isotopomers , formate , chemistry , metabolic pathway , metabolome , yield (engineering) , metabolomics , biochemistry , isotopic labeling , flux (metallurgy) , metabolism , metabolite , chromatography , organic chemistry , catalysis , materials science , molecule , metallurgy

Herein we report a study combining metabolomics and mass isotopomer analysis used for investigation of the biochemical fate of γ-hydroxybutyric acid (GHB). Using various (13)C incorporation labeling patterns into GHB, we have discovered that GHB is catabolized by previously unknown processes that include (i) direct β-oxidation to acetyl-CoA and glycolate, (ii) α-oxidation to 3-hydroxypropionyl-CoA and formate, and (iii) cleavage of C-4 to yield 3-hydroxypropionate and CO2. We further utilized the unique attributes of our labeling patterns and the resultant isotopomers to quantitate relative flux down the identified pathways.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research