Open AccessEngineering Transaldolase in Pichia stipitis to Improve Bioethanol Production
Author(s) -
Shan-He Chen,
DerRen Hwang,
GanHong Chen,
NingShian Hsu,
YingTa Wu,
TsungLin Li,
ChiHuey Wong
Publication year - 2011
Publication title -
acs chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.899
H-Index - 111
eISSN - 1554-8937
pISSN - 1554-8929
DOI - 10.1021/cb200396b
Subject(s) - transaldolase , pichia stipitis , xylose , bioconversion , pentose , ethanol fuel , fermentation , biochemistry , yield (engineering) , chemostat , chemistry , pentose phosphate pathway , food science , biology , enzyme , materials science , genetics , glycolysis , bacteria , metallurgy
In our effort to improve the efficiency and yield of xylose-to-ethanol bioconversion in Pichia stipitis, the transaldolase (TAL) in the pentose phosphate pathway was identified as a rate-limiting enzyme for improvement. A mutant containing the Q263R change was first obtained by directed evolution with 5-fold increase of activity, which was then incorporated into P. stipitesvia the pYDS vector to produce a genetically stable strain for fermentation on xylose. In comparison with the parental strain, TAL-Q263R(+) increases ethanol prodcution by 36% and 100% as measured by volumetric production rate and specific production rate, respectively. Thus improving the transaldolase activity in P. stipitis can significantly increase the rate and yield of xylose conversion to ethanol.
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