Automatic Structure Determination of Regular Polysaccharides Based Solely on NMR Spectroscopy | Zendy

Magnus Lundborg | Zendy; Carolina Fontana | Zendy; Göran Widmalm | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Automatic Structure Determination of Regular Polysaccharides Based Solely on NMR Spectroscopy

Author(s) -

Magnus Lundborg,

Carolina Fontana,

Göran Widmalm

Publication year - 2011

Publication title -

biomacromolecules

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.689

H-Index - 220

eISSN - 1526-4602

pISSN - 1525-7797

DOI - 10.1021/bm201169y

Subject(s) - nuclear magnetic resonance spectroscopy , chemistry , polysaccharide , spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , chemical structure , transverse relaxation optimized spectroscopy , analytical chemistry (journal) , fluorine 19 nmr , organic chemistry , stereochemistry , physics , quantum mechanics

The structural analysis of polysaccharides requires that the sugar components and their absolute configurations are determined. We here show that this can be performed based on NMR spectroscopy by utilizing butanolysis with (+)- and (-)-2-butanol that gives the corresponding 2-butyl glycosides with characteristic (1)H and (13)C NMR chemical shifts. The subsequent computer-assisted structural determination by CASPER can then be based solely on NMR data in a fully automatic way as shown and implemented herein. The method is additionally advantageous in that reference data only have to be prepared once and from a user's point of view only the unknown sample has to be derivatized for use in CASPER.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research