Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair | Zendy

Evgenia N. Nikolova | Zendy; Frederick Stull | Zendy; Hashim M. AlHashimi | Zendy

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Guanine to Inosine Substitution Leads to Large Increases in the Population of a Transient G·C Hoogsteen Base Pair

Author(s) -

Evgenia N. Nikolova,

Frederick Stull,

Hashim M. AlHashimi

Publication year - 2014

Publication title -

biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.43

H-Index - 253

eISSN - 1520-4995

pISSN - 0006-2960

DOI - 10.1021/bi5011909

Subject(s) - guanine , inosine , transient (computer programming) , base (topology) , chemistry , substitution (logic) , population , stereochemistry , biochemistry , mathematics , nucleotide , computer science , enzyme , medicine , environmental health , programming language , operating system , mathematical analysis , gene

We recently showed that Watson-Crick base pairs in canonical duplex DNA exist in dynamic equilibrium with G(syn)·C+ and A(syn)·T Hoogsteen base pairs that have minute populations of ∼1%. Here, using nuclear magnetic resonance R1ρ relaxation dispersion, we show that substitution of guanine with the naturally occurring base inosine results in an ∼17-fold increase in the population of transient Hoogsteen base pairs, which can be rationalized by the loss of a Watson-Crick hydrogen bond. These results provide further support for transient Hoogsteen base pairs and demonstrate that their population can increase significantly upon damage or chemical modification of the base.

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