Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina | Zendy

Thomas R. Sundermeier | Zendy; Hui Jin | Zendy; Matthew L. Kleinjan | Zendy; Debarshi Mustafi | Zendy; Donny D. Licatalosi | Zendy; Krzysztof Palczewski | Zendy

Open Access

Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

Author(s) -

Thomas R. Sundermeier,

Hui Jin,

Matthew L. Kleinjan,

Debarshi Mustafi,

Donny D. Licatalosi,

Krzysztof Palczewski

Publication year - 2014

Publication title -

biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.43

H-Index - 253

eISSN - 1520-4995

pISSN - 0006-2960

DOI - 10.1021/bi500966b

Subject(s) - argonaute , biology , microrna , gene , retina , immunoprecipitation , regulation of gene expression , genetics , computational biology , microbiology and biotechnology , rna interference , rna , neuroscience

Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 high-confidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases.

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