Crystal Structure of DNA Polymerase β with DNA Containing the Base Lesion Spiroiminodihydantoin in a Templating Position | Zendy

Brian E. Eckenroth | Zendy; Aaron M. Fleming | Zendy; Joann B. Sweasy | Zendy; Cynthia J. Burrows | Zendy; Sylvie Doublié | Zendy

Open Access

Crystal Structure of DNA Polymerase β with DNA Containing the Base Lesion Spiroiminodihydantoin in a Templating Position

Author(s) -

Brian E. Eckenroth,

Aaron M. Fleming,

Joann B. Sweasy,

Cynthia J. Burrows,

Sylvie Doublié

Publication year - 2014

Publication title -

biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.43

H-Index - 253

eISSN - 1520-4995

pISSN - 0006-2960

DOI - 10.1021/bi500270e

Subject(s) - dna , duplex (building) , base pair , crystallography , chemistry , dna polymerase , crystal structure , polymerase , ring (chemistry) , dna clamp , context (archaeology) , biophysics , stereochemistry , polymerase chain reaction , biology , gene , biochemistry , reverse transcriptase , organic chemistry , paleontology

The first high-resolution crystal structure of spiroiminodihydantoin (dSp1) was obtained in the context of the DNA polymerase β active site and reveals two areas of significance. First, the structure verifies the recently determined S configuration at the spirocyclic carbon. Second, the distortion of the DNA duplex is similar to that of the single-oxidation product 8-oxoguanine. For both oxidized lesions, adaptation of the syn conformation results in similar backbone distortions in the DNA duplex. The resulting conformation positions the dSp1 A-ring as the base-pairing face whereas the B-ring of dSp1 protrudes into the major groove.

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