Head-group conformation in phospholipids: a 31P nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers | Zendy

Robert G. Griffin | Zendy; L. Powers | Zendy; P. S. Pershan | Zendy

Open Access

Head-group conformation in phospholipids: a ³¹P nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers

Author(s) -

Robert G. Griffin,

L. Powers,

P. S. Pershan

Publication year - 1978

Publication title -

biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.43

H-Index - 253

eISSN - 1520-4995

pISSN - 0006-2960

DOI - 10.1021/bi00607a004

Subject(s) - dipalmitoylphosphatidylcholine , nuclear magnetic resonance , group (periodic table) , chemistry , head (geology) , magnetic resonance imaging , lipid bilayer , crystallography , phospholipid , membrane , physics , phosphatidylcholine , medicine , biochemistry , organic chemistry , biology , paleontology , radiology

Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC.H2O multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the O-P-O plane of the phosphate, where the O's are the nonesterified oxygens of the phosphodiester, is tilted at 47 +/- 5 degrees with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.

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